Extended phage locus typing of Salmonella enterica serovar Typhimurium, using multiplex PCR-based reverse line blot hybridization
نویسندگان
چکیده
منابع مشابه
Detection of Salmonella enterica Serovar Typhimurium from Avians Using Multiplex-PCR
Salmonella enterica serovar Typhimurium and S.enterica serovar Enteritidis are the most frequently isolated serovars from food-borne diseases throughout the world. According to their antigenic profiles, salmonella shows different disease syndromes and host specificities. It is necessary and important to discriminate salmonella serovars from each other in order to ensure that each pathogen and i...
متن کاملSalmonella enterica serovar Typhimurium typing by prophage-specific PCR.
Recent data from microarray analysis have shown that integrated prophages are the most frequent sources of genomic variation between different strains of Salmonella enterica serovar Typhimurium (S. Typhimurium). This led us to hypothesize that PCR detection of the integrated prophages might be an efficient typing tool that could be used as an alternative to PFGE. In this study, we optimized fou...
متن کاملMultiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Multiplex PCR/Reverse Line Blot Hybridization assay allows the detection of up to 43 molecular targets in 43 samples using one multiplex PCR reaction followed by probe hybridization on a nylon membrane, which is re-usable. Probes are 5' amine modified to allow fixation to the membrane. Primers are 5' biotin modified which allows detection of hybridized PCR products using streptavidin-peroxidase...
متن کاملMultilocus Sequence Typing of the Clinical Isolates of Salmonella Enterica Serovar Typhimurium in Tehran Hospitals
Background: Salmonella enterica serovar Typhimurium is one of the most important serovars of Salmonella enterica and is associated with human salmonellosis worldwide. Many epidemiological studies have focused on the characteristics of Salmonella Typhimurium in many countries as well as in Asia. This study was conducted to investigate the genetic characteristics of Salmonella Typhimurium using m...
متن کاملdetection of salmonella enterica serovar typhimurium from avians using multiplex-pcr
salmonella enterica serovar typhimurium and s.enterica serovar enteritidis are the most frequently isolated serovars from food-borne diseases throughout the world. according to their antigenic profiles, salmonella shows different disease syndromes and host specificities. it is necessary and important to discriminate salmonella serovars from each other in order to ensure that each pathogen and i...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2008
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.47766-0